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Assay Detail

CHEMBL4384330

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Hedgehog signaling pathway in mouse ASZ001 cells assessed as reduction in oxysterol-induced Gli1 mRNA expression after 48 hrs by q-PCR analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis and evaluation of third generation vitamin D3 analogues as inhibitors of Hedgehog signaling.
Maschinot CA, Chau LQ, Wechsler-Reya RJ, Hadden MK.
Eur J Med Chem (2019) 162 : 495 -506
PubMed 30471551 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.65 9.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL197589 LG-190178 1 9.64
CHEMBL473417 VISMODEGIB 4.0 1 7.40
CHEMBL846 CALCITRIOL 4.0 1 7.05
CHEMBL4459512 1 7.00
CHEMBL4580825 1 6.70
CHEMBL4582806 1 6.52
CHEMBL4522782 1 6.40
CHEMBL1040 CALCIFEDIOL ANHYDROUS 4.0 1 6.30
CHEMBL4449816 1 6.30
CHEMBL4542767 1 6.22
CHEMBL4459996 1 6.22
CHEMBL4524451 1 6.16
CHEMBL4439766 1 6.00
CHEMBL4443970 1 5.92
CHEMBL4438180 1 5.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL197589 LG-190178 IC50 = 0.23 nM 9.64
CHEMBL473417 VISMODEGIB IC50 = 40.0 nM 7.40
CHEMBL846 CALCITRIOL IC50 = 89.1 nM 7.05
CHEMBL4459512 IC50 = 100.0 nM 7.00
CHEMBL4580825 IC50 = 200.0 nM 6.70
CHEMBL4582806 IC50 = 300.0 nM 6.52
CHEMBL4522782 IC50 = 400.0 nM 6.40
CHEMBL1040 CALCIFEDIOL ANHYDROUS IC50 = 500.0 nM 6.30
CHEMBL4449816 IC50 = 500.0 nM 6.30
CHEMBL4542767 IC50 = 600.0 nM 6.22
CHEMBL4459996 IC50 = 600.0 nM 6.22
CHEMBL4524451 IC50 = 700.0 nM 6.16
CHEMBL4439766 IC50 = 1000.0 nM 6.00
CHEMBL4443970 IC50 = 1200.0 nM 5.92
CHEMBL4438180 IC50 = 1300.0 nM 5.89