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Assay Detail

CHEMBL4384349

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Hedgehog signaling pathway in Sufu-deficient mouse C3H10T1/2 cells assessed as reduction in oxysterol-induced Gli1 mRNA expression after 24 hrs by q-PCR analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis and evaluation of third generation vitamin D3 analogues as inhibitors of Hedgehog signaling.
Maschinot CA, Chau LQ, Wechsler-Reya RJ, Hadden MK.
Eur J Med Chem (2019) 162 : 495 -506
PubMed 30471551 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.37 5.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4580825 1 5.54
CHEMBL3408015 1 5.51
CHEMBL846 CALCITRIOL 4.0 1 5.07
CHEMBL1042 CHOLECALCIFEROL 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4580825 IC50 = 2900.0 nM 5.54
CHEMBL3408015 IC50 = 3100.0 nM 5.51
CHEMBL846 CALCITRIOL IC50 = 8500.0 nM 5.07
CHEMBL1042 CHOLECALCIFEROL IC50 > 10000.0 nM -