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Assay Detail

CHEMBL4385972

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human FLT3 (564 to end residues) using EAIYAAPFAKKK as substrate measured after 40 mins in presence of [gamma-33P]-ATP by scintillation counting analysis
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of Pyrrolo[2,3- d]pyrimidine-Based Derivatives as Potent and Orally Effective Fms-like Tyrosine Receptor Kinase 3 (FLT3) Inhibitors for Treating Acute Myelogenous Leukemia.
Yuan X, Chen Y, Zhang W, He J, Lei L, Tang M, Liu J, Li M, Dou C, Yang T, Yang L, Yang S, Wei Y, Peng A, Niu T, Xiang M, Ye H, Chen L.
J Med Chem (2019) 62 : 4158 -4173
PubMed 30939008 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 7.66 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4551114 1 8.40
CHEMBL4517711 1 8.22
CHEMBL4435457 1 8.22
CHEMBL4439404 1 8.15
CHEMBL4572972 1 8.15
CHEMBL4557634 1 8.15
CHEMBL576982 QUIZARTINIB 4.0 1 8.13
CHEMBL4572804 1 8.05
CHEMBL4440175 1 8.05
CHEMBL4441629 1 8.05
CHEMBL4459941 1 7.89
CHEMBL4441875 1 7.82
CHEMBL4541344 1 7.80
CHEMBL4475375 1 7.77
CHEMBL4454804 1 7.77
CHEMBL4461531 1 7.72
CHEMBL4435360 1 7.70
CHEMBL4515336 1 7.70
CHEMBL4575519 1 7.68
CHEMBL4472511 1 7.66
CHEMBL4526888 1 7.60
CHEMBL4539069 1 7.58
CHEMBL4438587 1 7.58
CHEMBL4569015 1 7.52
CHEMBL4586009 1 7.51
CHEMBL4529106 1 7.50
CHEMBL4532475 1 7.50
CHEMBL4449006 1 7.47
CHEMBL4553847 1 7.43
CHEMBL4591906 1 7.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4551114 IC50 = 4.0 nM 8.40
CHEMBL4517711 IC50 = 6.0 nM 8.22
CHEMBL4435457 IC50 = 6.0 nM 8.22
CHEMBL4439404 IC50 = 7.0 nM 8.15
CHEMBL4572972 IC50 = 7.0 nM 8.15
CHEMBL4557634 IC50 = 7.0 nM 8.15
CHEMBL576982 QUIZARTINIB IC50 = 7.4 nM 8.13
CHEMBL4572804 IC50 = 9.0 nM 8.05
CHEMBL4440175 IC50 = 9.0 nM 8.05
CHEMBL4441629 IC50 = 9.0 nM 8.05
CHEMBL4459941 IC50 = 13.0 nM 7.89
CHEMBL4441875 IC50 = 15.0 nM 7.82
CHEMBL4541344 IC50 = 16.0 nM 7.80
CHEMBL4475375 IC50 = 17.0 nM 7.77
CHEMBL4454804 IC50 = 17.0 nM 7.77
CHEMBL4461531 IC50 = 19.0 nM 7.72
CHEMBL4435360 IC50 = 20.0 nM 7.70
CHEMBL4515336 IC50 = 20.0 nM 7.70
CHEMBL4575519 IC50 = 21.0 nM 7.68
CHEMBL4472511 IC50 = 22.0 nM 7.66
CHEMBL4526888 IC50 = 25.0 nM 7.60
CHEMBL4438587 IC50 = 26.0 nM 7.58
CHEMBL4539069 IC50 = 26.0 nM 7.58
CHEMBL4569015 IC50 = 30.0 nM 7.52
CHEMBL4586009 IC50 = 31.0 nM 7.51
CHEMBL4529106 IC50 = 32.0 nM 7.50
CHEMBL4532475 IC50 = 32.0 nM 7.50
CHEMBL4449006 IC50 = 34.0 nM 7.47
CHEMBL4553847 IC50 = 37.0 nM 7.43
CHEMBL4591906 IC50 = 38.0 nM 7.42
CHEMBL4483045 IC50 = 46.0 nM 7.34
CHEMBL4439044 IC50 = 49.0 nM 7.31
CHEMBL4558160 IC50 = 51.0 nM 7.29
CHEMBL4553033 IC50 = 64.0 nM 7.19
CHEMBL4442278 IC50 = 70.0 nM 7.16
CHEMBL4457542 IC50 = 69.0 nM 7.16
CHEMBL4551816 IC50 = 86.0 nM 7.07
CHEMBL4533761 IC50 = 105.0 nM 6.98
CHEMBL4468986 IC50 = 113.0 nM 6.95
CHEMBL4449318 IC50 - -