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Assay Detail

CHEMBL4386553

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of Nrf2 in human BEAS2B cells assessed as increase in NQO1 enzymatic activity measured after 48 hrs by MTT assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nuclear factor erythroid 2-related factor 2 (CHEMBL1075094)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Activity and Structure-Conformation Relationships of Aryl Propionic Acid Inhibitors of the Kelch-like ECH-Associated Protein 1/Nuclear Factor Erythroid 2-Related Factor 2 (KEAP1/NRF2) Protein-Protein Interaction.
Heightman TD, Callahan JF, Chiarparin E, Coyle JE, Griffiths-Jones C, Lakdawala AS, McMenamin R, Mortenson PN, Norton D, Peakman TM, Rich SJ, Richardson C, Rumsey WL, Sanchez Y, Saxty G, Willems HMG, Wolfe L, Woolford AJ, Wu Z, Yan H, Kerns JK, Davies TG.
J Med Chem (2019) 62 : 4683 -4702
PubMed 30973731 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 6.37 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4450921 1 7.96
CHEMBL4447060 1 7.52
CHEMBL4578025 1 7.08
CHEMBL4540327 1 6.24
CHEMBL4457466 1 6.12
CHEMBL4554007 1 6.07
CHEMBL4567490 1 5.85
CHEMBL4464511 1 5.80
CHEMBL4454051 1 5.54
CHEMBL4473977 1 5.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4450921 EC50 = 11.0 nM 7.96
CHEMBL4447060 EC50 = 30.0 nM 7.52
CHEMBL4578025 EC50 = 83.0 nM 7.08
CHEMBL4540327 EC50 = 570.0 nM 6.24
CHEMBL4457466 EC50 = 760.0 nM 6.12
CHEMBL4554007 EC50 = 850.0 nM 6.07
CHEMBL4567490 EC50 = 1400.0 nM 5.85
CHEMBL4464511 EC50 = 1600.0 nM 5.80
CHEMBL4454051 EC50 = 2900.0 nM 5.54
CHEMBL4473977 EC50 = 3400.0 nM 5.47