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Assay Detail

CHEMBL4386594

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Pseudomonas aeruginosa PAO1 N-terminal His-tagged AmpC expressed in Escherichia coli BL21(DE3) assessed as residual enzyme activity using FC5 as substrate pre-incubated for 10 mins followed by substrate addition by fluorescence method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa PAO1
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Profiling interactions of vaborbactam with metallo-β-lactamases.
Langley GW, Cain R, Tyrrell JM, Hinchliffe P, Calvopiña K, Tooke CL, Widlake E, Dowson CG, Spencer J, Walsh TR, Schofield CJ, Brem J.
Bioorg Med Chem Lett (2019) 29 : 1981 -1984
PubMed 31171422 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.92 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4533632 1 6.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4533632 IC50 = 120.0 nM 6.92