Assay Detail
Binding
CHEMBL4386711
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NS5A in HCV genotype 2a JFH1 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus subtype 2a |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Discovery of velpatasvir (GS-5816): A potent pan-genotypic HCV NS5A inhibitor in the single-tablet regimens Vosevi<sup>®</sup> and Epclusa<sup>®</sup>.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 12 | 9.21 | 10.74 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4473265 | — | — | 1 | 10.74 |
| CHEMBL2374220 | LEDIPASVIR | 4.0 | 1 | 7.68 |
| CHEMBL3545062 | VELPATASVIR | 4.0 | 1 | - |
| CHEMBL4516340 | — | — | 1 | - |
| CHEMBL4468617 | — | — | 1 | - |
| CHEMBL4457264 | — | — | 1 | - |
| CHEMBL4574152 | — | — | 1 | - |
| CHEMBL4467926 | — | — | 1 | - |
| CHEMBL4557789 | — | — | 1 | - |
| CHEMBL4587426 | — | — | 1 | - |
| CHEMBL4547877 | — | — | 1 | - |
| CHEMBL4437858 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4473265 | — | EC50 | = | 0.018 | nM | 10.74 |
| CHEMBL2374220 | LEDIPASVIR | EC50 | = | 21.0 | nM | 7.68 |
| CHEMBL3545062 | VELPATASVIR | EC50 | = | 0.008 | nM | - |
| CHEMBL4516340 | — | EC50 | = | 0.004 | nM | - |
| CHEMBL4468617 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4457264 | — | EC50 | = | 0.008 | nM | - |
| CHEMBL4574152 | — | EC50 | = | 0.005 | nM | - |
| CHEMBL4467926 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4557789 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4587426 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4547877 | — | EC50 | = | 0.007 | nM | - |
| CHEMBL4437858 | — | EC50 | = | 0.004 | nM | - |