Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4386713

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NS5A in HCV genotype 2b MD2b-1 infected in human HuH7 replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 2b
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Nonstructural protein 5A (CHEMBL3307224)
Type SINGLE PROTEIN
Organism Hepatitis C virus

Publication

Discovery of velpatasvir (GS-5816): A potent pan-genotypic HCV NS5A inhibitor in the single-tablet regimens Vosevi<sup>®</sup> and Epclusa<sup>®</sup>.
Link JO, Taylor JG, Trejo-Martin A, Kato D, Katana AA, Krygowski ES, Yang ZY, Zipfel S, Cottell JJ, Bacon EM, Tran CV, Yang CY, Wang Y, Wang KW, Zhao G, Cheng G, Tian Y, Gong R, Lee YJ, Yu M, Gorman E, Mogalian E, Perry JK.
Bioorg Med Chem Lett (2019) 29 : 2415 -2427
PubMed 31230974 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 9.84 10.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4557789 1 10.82
CHEMBL4437858 1 10.82
CHEMBL4473265 1 10.57
CHEMBL4547877 1 10.55
CHEMBL4587426 1 10.34
CHEMBL4449384 1 10.20
CHEMBL4450375 1 9.88
CHEMBL4565239 1 9.87
CHEMBL4583723 1 9.80
CHEMBL4576714 1 9.78
CHEMBL4440405 1 9.74
CHEMBL4542363 1 9.58
CHEMBL4588741 1 9.54
CHEMBL2374220 LEDIPASVIR 4.0 1 6.28
CHEMBL3545062 VELPATASVIR 4.0 1 -
CHEMBL4467926 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4557789 EC50 = 0.015 nM 10.82
CHEMBL4437858 EC50 = 0.015 nM 10.82
CHEMBL4473265 EC50 = 0.027 nM 10.57
CHEMBL4547877 EC50 = 0.028 nM 10.55
CHEMBL4587426 EC50 = 0.046 nM 10.34
CHEMBL4449384 EC50 = 0.063 nM 10.20
CHEMBL4450375 EC50 = 0.132 nM 9.88
CHEMBL4565239 EC50 = 0.135 nM 9.87
CHEMBL4583723 EC50 = 0.157 nM 9.80
CHEMBL4576714 EC50 = 0.166 nM 9.78
CHEMBL4440405 EC50 = 0.18 nM 9.74
CHEMBL4542363 EC50 = 0.265 nM 9.58
CHEMBL4588741 EC50 = 0.29 nM 9.54
CHEMBL2374220 LEDIPASVIR EC50 = 530.0 nM 6.28
CHEMBL3545062 VELPATASVIR EC50 = 0.006 nM -
CHEMBL4467926 EC50 = 0.009 nM -