Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4386779

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NS5A in HCV genotype 3a S52 infected in human HuH7 transient chimeric replicon cells assessed as reduction in viral replication after 3 days by luciferase reporter gene assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 3a
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Nonstructural protein 5A (CHEMBL3307224)
Type SINGLE PROTEIN
Organism Hepatitis C virus

Publication

Discovery of velpatasvir (GS-5816): A potent pan-genotypic HCV NS5A inhibitor in the single-tablet regimens Vosevi<sup>®</sup> and Epclusa<sup>®</sup>.
Link JO, Taylor JG, Trejo-Martin A, Kato D, Katana AA, Krygowski ES, Yang ZY, Zipfel S, Cottell JJ, Bacon EM, Tran CV, Yang CY, Wang Y, Wang KW, Zhao G, Cheng G, Tian Y, Gong R, Lee YJ, Yu M, Gorman E, Mogalian E, Perry JK.
Bioorg Med Chem Lett (2019) 29 : 2415 -2427
PubMed 31230974 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 10.46 10.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4453135 1 10.92
CHEMBL4467926 1 10.77
CHEMBL4450375 1 10.74
CHEMBL4587426 1 10.74
CHEMBL4449384 1 10.70
CHEMBL4437858 1 10.70
CHEMBL4565239 1 10.62
CHEMBL4547877 1 10.60
CHEMBL4557789 1 10.60
CHEMBL4542363 1 10.32
CHEMBL4473265 1 10.30
CHEMBL4436929 1 10.26
CHEMBL4576714 1 10.24
CHEMBL4583723 1 10.21
CHEMBL4588741 1 9.92
CHEMBL4440405 1 9.69
CHEMBL4574152 1 -
CHEMBL4457264 1 -
CHEMBL4468617 1 -
CHEMBL4516340 1 -
CHEMBL4536960 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4453135 EC50 = 0.012 nM 10.92
CHEMBL4467926 EC50 = 0.017 nM 10.77
CHEMBL4450375 EC50 = 0.018 nM 10.74
CHEMBL4587426 EC50 = 0.018 nM 10.74
CHEMBL4449384 EC50 = 0.02 nM 10.70
CHEMBL4437858 EC50 = 0.02 nM 10.70
CHEMBL4565239 EC50 = 0.024 nM 10.62
CHEMBL4547877 EC50 = 0.025 nM 10.60
CHEMBL4557789 EC50 = 0.025 nM 10.60
CHEMBL4542363 EC50 = 0.048 nM 10.32
CHEMBL4473265 EC50 = 0.05 nM 10.30
CHEMBL4436929 EC50 = 0.055 nM 10.26
CHEMBL4576714 EC50 = 0.058 nM 10.24
CHEMBL4583723 EC50 = 0.061 nM 10.21
CHEMBL4588741 EC50 = 0.12 nM 9.92
CHEMBL4440405 EC50 = 0.206 nM 9.69
CHEMBL4574152 EC50 = 0.006 nM -
CHEMBL4457264 EC50 = 0.004 nM -
CHEMBL4468617 EC50 = 0.006 nM -
CHEMBL4516340 EC50 = 0.005 nM -
CHEMBL4536960 EC50 = 0.004 nM -