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Assay Detail

CHEMBL4388461

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant NAMPT (unknown origin) using NAM and PRPP as substrates incubated for 60 mins in presence of NMNAT1 measured at 5 mins interval for 30 mins by WST1 assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide phosphoribosyltransferase (CHEMBL1744525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of trans-3-(pyridin-3-yl)acrylamide-derived sulfamides as potent nicotinamide phosphoribosyltransferase (NAMPT) inhibitors for the potential treatment of cancer.
Zhang K, Ni Y, Chen J, Tu Z, Wu X, Chen D, Yao H, Jiang S.
Bioorg Med Chem Lett (2019) 29 : 1502 -1506
PubMed 30992165 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.69 8.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4475995 1 8.29
CHEMBL566757 DAPORINAD 2.0 1 8.17
CHEMBL4579284 1 8.04
CHEMBL4561168 1 8.02
CHEMBL4453985 1 8.01
CHEMBL4453544 1 8.01
CHEMBL4540043 1 7.98
CHEMBL4527968 1 7.97
CHEMBL4578113 1 7.93
CHEMBL4459869 1 7.91
CHEMBL4522903 1 7.88
CHEMBL4451832 1 7.84
CHEMBL4451262 1 7.79
CHEMBL4515592 1 7.78
CHEMBL4562385 1 7.75
CHEMBL4470144 1 7.61
CHEMBL4516605 1 7.55
CHEMBL4518604 1 7.50
CHEMBL4454520 1 7.50
CHEMBL4454348 1 6.63
CHEMBL4567362 1 5.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4475995 IC50 = 5.08 nM 8.29
CHEMBL566757 DAPORINAD IC50 = 6.8 nM 8.17
CHEMBL4579284 IC50 = 9.2 nM 8.04
CHEMBL4561168 IC50 = 9.47 nM 8.02
CHEMBL4453985 IC50 = 9.7 nM 8.01
CHEMBL4453544 IC50 = 9.78 nM 8.01
CHEMBL4540043 IC50 = 10.53 nM 7.98
CHEMBL4527968 IC50 = 10.75 nM 7.97
CHEMBL4578113 IC50 = 11.63 nM 7.93
CHEMBL4459869 IC50 = 12.28 nM 7.91
CHEMBL4522903 IC50 = 13.09 nM 7.88
CHEMBL4451832 IC50 = 14.42 nM 7.84
CHEMBL4451262 IC50 = 16.41 nM 7.79
CHEMBL4515592 IC50 = 16.54 nM 7.78
CHEMBL4562385 IC50 = 17.77 nM 7.75
CHEMBL4470144 IC50 = 24.53 nM 7.61
CHEMBL4516605 IC50 = 28.39 nM 7.55
CHEMBL4518604 IC50 = 31.32 nM 7.50
CHEMBL4454520 IC50 = 31.54 nM 7.50
CHEMBL4454348 IC50 = 234.5 nM 6.63
CHEMBL4567362 IC50 = 5920.0 nM 5.23