Assay Detail
Binding
CHEMBL4390131
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Pneumocystis jirovecii DHFR expressed in Escherichia coli Rosetta-gami B (DE3) assessed as reduction in consumption of NADPH using 18 uM DHFA as substrate
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Pneumocystis jirovecii |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Development of substituted pyrido[3,2-d]pyrimidines as potent and selective dihydrofolate reductase inhibitors for pneumocystis pneumonia infection.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 6.95 | 8.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL7492 | PIRITREXIM | 2.0 | 1 | 8.80 |
| CHEMBL119 | TRIMETREXATE | 4.0 | 1 | 8.68 |
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | 6.92 |
| CHEMBL2382323 | — | — | 1 | 6.60 |
| CHEMBL2382314 | — | — | 1 | 6.52 |
| CHEMBL2382315 | — | — | 1 | 6.40 |
| CHEMBL2382321 | — | — | 1 | 6.40 |
| CHEMBL2382319 | — | — | 1 | 6.21 |
| CHEMBL2382325 | — | — | 1 | 6.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL7492 | PIRITREXIM | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL119 | TRIMETREXATE | IC50 | = | 2.1 | nM | 8.68 |
| CHEMBL22 | TRIMETHOPRIM | IC50 | = | 120.0 | nM | 6.92 |
| CHEMBL2382323 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL2382314 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL2382315 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL2382321 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL2382319 | — | IC50 | = | 620.0 | nM | 6.21 |
| CHEMBL2382325 | — | IC50 | = | 870.0 | nM | 6.06 |