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Assay Detail

CHEMBL4390132

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ADMET
Inhibition of recombinant human DHFR assessed as reduction in consumption of NADPH using 18 uM DHFA as substrate
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of substituted pyrido[3,2-d]pyrimidines as potent and selective dihydrofolate reductase inhibitors for pneumocystis pneumonia infection.
Shah K, Queener S, Cody V, Pace J, Gangjee A.
Bioorg Med Chem Lett (2019) 29 : 1874 -1880
PubMed 31176699 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.25 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL119 TRIMETREXATE 4.0 1 8.59
CHEMBL7492 PIRITREXIM 2.0 1 8.52
CHEMBL2382314 1 6.72
CHEMBL2382319 1 5.68
CHEMBL2382323 1 5.68
CHEMBL2382321 1 5.66
CHEMBL2382325 1 5.51
CHEMBL2382315 1 5.44
CHEMBL22 TRIMETHOPRIM 4.0 1 4.49

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL119 TRIMETREXATE IC50 = 2.6 nM 8.59
CHEMBL7492 PIRITREXIM IC50 = 3.0 nM 8.52
CHEMBL2382314 IC50 = 190.0 nM 6.72
CHEMBL2382319 IC50 = 2100.0 nM 5.68
CHEMBL2382323 IC50 = 2100.0 nM 5.68
CHEMBL2382321 IC50 = 2200.0 nM 5.66
CHEMBL2382325 IC50 = 3100.0 nM 5.51
CHEMBL2382315 IC50 = 3650.0 nM 5.44
CHEMBL22 TRIMETHOPRIM IC50 = 32200.0 nM 4.49