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Assay Detail

CHEMBL4390137

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of recombinant human DHFR assessed as reduction in consumption of NADPH using 9 uM DHFA as substrate
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of substituted pyrido[3,2-d]pyrimidines as potent and selective dihydrofolate reductase inhibitors for pneumocystis pneumonia infection.
Shah K, Queener S, Cody V, Pace J, Gangjee A.
Bioorg Med Chem Lett (2019) 29 : 1874 -1880
PubMed 31176699 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.87 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL7492 PIRITREXIM 2.0 1 8.70
CHEMBL4440383 1 6.04
CHEMBL140940 1 6.03
CHEMBL4449630 1 5.96
CHEMBL4588482 1 5.87
CHEMBL4589761 1 5.86
CHEMBL139188 1 5.82
CHEMBL4573954 1 5.80
CHEMBL4571736 1 5.80
CHEMBL4522888 1 5.74
CHEMBL4522334 1 5.65
CHEMBL141218 1 5.61
CHEMBL4462778 1 5.58
CHEMBL138419 1 5.50
CHEMBL4469864 1 5.38
CHEMBL22 TRIMETHOPRIM 4.0 1 4.61

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL7492 PIRITREXIM IC50 = 2.0 nM 8.70
CHEMBL4440383 IC50 = 903.0 nM 6.04
CHEMBL140940 IC50 = 942.0 nM 6.03
CHEMBL4449630 IC50 = 1098.0 nM 5.96
CHEMBL4588482 IC50 = 1338.0 nM 5.87
CHEMBL4589761 IC50 = 1373.0 nM 5.86
CHEMBL139188 IC50 = 1526.0 nM 5.82
CHEMBL4573954 IC50 = 1576.0 nM 5.80
CHEMBL4571736 IC50 = 1571.0 nM 5.80
CHEMBL4522888 IC50 = 1808.0 nM 5.74
CHEMBL4522334 IC50 = 2253.0 nM 5.65
CHEMBL141218 IC50 = 2459.0 nM 5.61
CHEMBL4462778 IC50 = 2626.0 nM 5.58
CHEMBL138419 IC50 = 3185.0 nM 5.50
CHEMBL4469864 IC50 = 4125.0 nM 5.38
CHEMBL22 TRIMETHOPRIM IC50 = 24500.0 nM 4.61