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Assay Detail

CHEMBL4391749

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human aromatase preincubated for 10 mins followed by substrate and beta-NADP+ addition and measured for 60 mins by fluorescence method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aromatase (CHEMBL1978)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational design of allosteric modulators of the aromatase enzyme: An unprecedented therapeutic strategy to fight breast cancer.
Spinello A, Martini S, Berti F, Pennati M, Pavlin M, Sgrignani J, Grazioso G, Colombo G, Zaffaroni N, Magistrato A.
Eur J Med Chem (2019) 168 : 253 -262
PubMed 30822713 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.26 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1444 LETROZOLE 4.0 1 8.00
CHEMBL4437198 1 5.29
CHEMBL1093458 ENDOXIFEN 3.0 1 5.21
CHEMBL4568316 1 4.96
CHEMBL4474808 1 4.68
CHEMBL4453737 1 4.66
CHEMBL1991089 1 4.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1444 LETROZOLE IC50 = 10.0 nM 8.00
CHEMBL4437198 IC50 = 5100.0 nM 5.29
CHEMBL1093458 ENDOXIFEN IC50 = 6100.0 nM 5.21
CHEMBL4568316 IC50 = 11000.0 nM 4.96
CHEMBL4474808 IC50 = 20900.0 nM 4.68
CHEMBL4453737 IC50 = 21700.0 nM 4.66
CHEMBL1991089 IC50 = 95300.0 nM 4.02