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Assay Detail

CHEMBL4392180

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Functional
Antagonist activity at human D4 receptor expressed in CHOK1 cells assessed as suppression of dopamine-induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins by Eu-cAMP tracer based TR-FRET assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(4) dopamine receptor (CHEMBL219)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dopamine D4 Receptor-Selective Compounds Reveal Structure-Activity Relationships that Engender Agonist Efficacy.
Keck TM, Free RB, Day MM, Brown SL, Maddaluna MS, Fountain G, Cooper C, Fallon B, Holmes M, Stang CT, Burkhardt R, Bonifazi A, Ellenberger MP, Newman AH, Sibley DR, Wu C, Boateng CA.
J Med Chem (2019) 62 : 3722 -3740
PubMed 30883109 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.77 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL216393 1 8.70
CHEMBL375596 1 7.17
CHEMBL4448320 1 7.08
CHEMBL4587925 1 6.34
CHEMBL4443054 1 6.33
CHEMBL4442548 1 5.88
CHEMBL4454068 1 5.79
CHEMBL4437912 1 5.72
CHEMBL4588808 1 5.67
CHEMBL4473652 1 5.60
CHEMBL4473792 1 5.51
CHEMBL4572245 1 5.33
CHEMBL4464861 1 5.20
CHEMBL4443848 1 5.15
CHEMBL4591118 1 4.40
CHEMBL4436479 1 4.15
CHEMBL4592753 1 4.06
CHEMBL4453069 1 -
CHEMBL4456754 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL216393 IC50 = 2.0 nM 8.70
CHEMBL375596 IC50 = 68.4 nM 7.17
CHEMBL4448320 IC50 = 82.7 nM 7.08
CHEMBL4587925 IC50 = 453.0 nM 6.34
CHEMBL4443054 IC50 = 463.0 nM 6.33
CHEMBL4442548 IC50 = 1311.0 nM 5.88
CHEMBL4454068 IC50 = 1629.0 nM 5.79
CHEMBL4437912 IC50 = 1908.0 nM 5.72
CHEMBL4588808 IC50 = 2120.0 nM 5.67
CHEMBL4473652 IC50 = 2521.0 nM 5.60
CHEMBL4473792 IC50 = 3064.0 nM 5.51
CHEMBL4572245 IC50 = 4701.0 nM 5.33
CHEMBL4464861 IC50 = 6343.0 nM 5.20
CHEMBL4443848 IC50 = 7059.0 nM 5.15
CHEMBL4591118 IC50 = 40000.0 nM 4.40
CHEMBL4436479 IC50 = 70157.0 nM 4.15
CHEMBL4592753 IC50 = 86493.0 nM 4.06
CHEMBL4453069 IC50 > 100000.0 nM -
CHEMBL4456754 IC50 > 100000.0 nM -