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Assay Detail

CHEMBL4392258

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Binding
Inhibition of recombinant SK2 (unknown origin) expressed in Sf9 insect cells using D-erythro-sphingosine as substrate measured in presence of [gamma32P]ATP by scintillation proximity assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sphingosine kinase 2 (CHEMBL3023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Topographical Mapping of Isoform-Selectivity Determinants for J-Channel-Binding Inhibitors of Sphingosine Kinases 1 and 2.
Adams DR, Tawati S, Berretta G, Rivas PL, Baiget J, Jiang Z, Alsfouk A, Mackay SP, Pyne NJ, Pyne S.
J Med Chem (2019) 62 : 3658 -3676
PubMed 30889352 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 1 5.89 5.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3086782 1 5.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3086782 Ki = 1300.0 nM 5.89