Assay Detail
Binding
CHEMBL4392267
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human C-terminal His-tagged soluble form of CD73 expressed in baculovirus infected Sf9 insect cells using [2,8-3H]AMP as substrate measured after 25 mins by scintillation counting method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Structure-Activity Relationship of Purine and Pyrimidine Nucleotides as Ecto-5'-Nucleotidase (CD73) Inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 6 | 8.07 | 8.34 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4483379 | — | — | 1 | 8.34 |
| CHEMBL3606064 | — | — | 1 | 8.27 |
| CHEMBL4443094 | — | — | 1 | 8.16 |
| CHEMBL4443977 | — | — | 1 | 7.97 |
| CHEMBL4551648 | — | — | 1 | 7.85 |
| CHEMBL4470616 | — | — | 1 | 7.80 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4483379 | — | Ki | = | 4.58 | nM | 8.34 |
| CHEMBL3606064 | — | Ki | = | 5.33 | nM | 8.27 |
| CHEMBL4443094 | — | Ki | = | 6.88 | nM | 8.16 |
| CHEMBL4443977 | — | Ki | = | 10.6 | nM | 7.97 |
| CHEMBL4551648 | — | Ki | = | 14.0 | nM | 7.85 |
| CHEMBL4470616 | — | Ki | = | 15.9 | nM | 7.80 |