Assay Detail
Binding
CHEMBL4394392
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HCV NS5B RNA-dependent RNA polymerase activity assessed as reduction in radiolabeled-UTP incorporation into RNA using cIRES template measured after 2 hrs by microscintillation counting method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
RNA-directed RNA polymerase (CHEMBL4296320) ↗| Type | SINGLE PROTEIN |
| Organism | Hepacivirus hominis |
Publication
Synthesis and Anti-HCV Activities of 4'-Fluoro-2'-Substituted Uridine Triphosphates and Nucleotide Prodrugs: Discovery of 4'-Fluoro-2'- C-methyluridine 5'-Phosphoramidate Prodrug (AL-335) for the Treatment of Hepatitis C Infection.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 6.44 | 7.57 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4127092 | — | — | 1 | 7.57 |
| CHEMBL4125966 | — | — | 1 | 7.41 |
| CHEMBL4555315 | — | — | 1 | 6.96 |
| CHEMBL4127030 | ADAFOSBUVIR 5'-TRIPHOSPHATE | -1.0 | 1 | 6.85 |
| CHEMBL4543543 | — | — | 1 | 6.82 |
| CHEMBL4592496 | — | — | 1 | 6.72 |
| CHEMBL4592592 | — | — | 1 | 6.55 |
| CHEMBL4088430 | — | — | 1 | 6.52 |
| CHEMBL4127921 | — | — | 1 | 6.48 |
| CHEMBL4454742 | — | — | 1 | 6.47 |
| CHEMBL4440367 | — | — | 1 | 6.07 |
| CHEMBL4522072 | — | — | 1 | 5.19 |
| CHEMBL4546003 | — | — | 1 | 4.08 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4127092 | — | IC50 | = | 27.0 | nM | 7.57 |
| CHEMBL4125966 | — | IC50 | = | 39.0 | nM | 7.41 |
| CHEMBL4555315 | — | IC50 | = | 110.0 | nM | 6.96 |
| CHEMBL4127030 | ADAFOSBUVIR 5'-TRIPHOSPHATE | IC50 | = | 140.0 | nM | 6.85 |
| CHEMBL4543543 | — | IC50 | = | 150.0 | nM | 6.82 |
| CHEMBL4592496 | — | IC50 | = | 190.0 | nM | 6.72 |
| CHEMBL4592592 | — | IC50 | = | 280.0 | nM | 6.55 |
| CHEMBL4088430 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL4127921 | — | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL4454742 | — | IC50 | = | 340.0 | nM | 6.47 |
| CHEMBL4440367 | — | IC50 | = | 860.0 | nM | 6.07 |
| CHEMBL4522072 | — | IC50 | = | 6400.0 | nM | 5.19 |
| CHEMBL4546003 | — | IC50 | = | 84000.0 | nM | 4.08 |