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Assay Detail

CHEMBL4397429

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged human PDE5A1 expressed in baculovirus infected sf9 cells using [3H]cGMP as substrate measured after 30 mins by scintillation proximity assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL1827)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pharmacokinetics-Driven Optimization of 4(3 H)-Pyrimidinones as Phosphodiesterase Type 5 Inhibitors Leading to TPN171, a Clinical Candidate for the Treatment of Pulmonary Arterial Hypertension.
Wang Z, Jiang X, Zhang X, Tian G, Yang R, Wu J, Zou X, Liu Z, Yang X, Wu C, Shi J, Li J, Suo J, Wang Y, Zhang R, Xu Z, Gong X, He Y, Zhu W, Aisa HA, Jiang H, Xu Y, Shen J.
J Med Chem (2019) 62 : 4979 -4990
PubMed 31021628 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.85 9.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4455533 1 9.27
CHEMBL4462192 1 9.21
CHEMBL4474634 1 9.10
CHEMBL4444940 1 9.08
CHEMBL4467125 1 9.04
CHEMBL4468620 1 9.00
CHEMBL4559488 1 8.92
CHEMBL4483406 1 8.91
CHEMBL4572238 1 8.84
CHEMBL4435186 1 8.81
CHEMBL2180945 1 8.80
CHEMBL4455861 1 8.79
CHEMBL4440789 1 8.77
CHEMBL4582221 1 8.73
CHEMBL4539785 1 8.70
CHEMBL779 TADALAFIL 4.0 1 8.63
CHEMBL192 SILDENAFIL 4.0 1 8.37
CHEMBL4522391 1 8.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4455533 IC50 = 0.54 nM 9.27
CHEMBL4462192 IC50 = 0.62 nM 9.21
CHEMBL4474634 IC50 = 0.79 nM 9.10
CHEMBL4444940 IC50 = 0.83 nM 9.08
CHEMBL4467125 IC50 = 0.92 nM 9.04
CHEMBL4468620 IC50 = 1.01 nM 9.00
CHEMBL4559488 IC50 = 1.21 nM 8.92
CHEMBL4483406 IC50 = 1.23 nM 8.91
CHEMBL4572238 IC50 = 1.46 nM 8.84
CHEMBL4435186 IC50 = 1.55 nM 8.81
CHEMBL2180945 IC50 = 1.6 nM 8.80
CHEMBL4455861 IC50 = 1.61 nM 8.79
CHEMBL4440789 IC50 = 1.69 nM 8.77
CHEMBL4582221 IC50 = 1.86 nM 8.73
CHEMBL4539785 IC50 = 2.02 nM 8.70
CHEMBL779 TADALAFIL IC50 = 2.35 nM 8.63
CHEMBL192 SILDENAFIL IC50 = 4.31 nM 8.37
CHEMBL4522391 IC50 = 5.33 nM 8.27