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Assay Detail

CHEMBL4397963

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human HDAC3/NCOR1 assessed as decrease in deacetylation of FLUOR DE LYS SIRT1 substrate preincubated for 10 mins followed by substrate addition and and measured after 30 mins by fluorescence based assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Histone deacetylase 3/NCoR1 (CHEMBL3038484)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Thioether-based 2-aminobenzamide derivatives: Novel HDAC inhibitors with potent in vitro and in vivo antitumor activity.
Yun F, Cheng C, Ullah S, He J, Zahi MR, Yuan Q.
Eur J Med Chem (2019) 176 : 195 -207
PubMed 31103900 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.63 6.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4446236 1 6.87
CHEMBL4450474 1 6.67
CHEMBL4087968 1 6.63
CHEMBL4437852 1 6.56
CHEMBL4437865 1 6.42
CHEMBL235191 TACEDINALINE 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4446236 IC50 = 136.0 nM 6.87
CHEMBL4450474 IC50 = 215.0 nM 6.67
CHEMBL4087968 IC50 = 234.0 nM 6.63
CHEMBL4437852 IC50 = 274.0 nM 6.56
CHEMBL4437865 IC50 = 381.0 nM 6.42
CHEMBL235191 TACEDINALINE IC50 > 32000.0 nM -