Assay Detail
Binding
CHEMBL4398138
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to human CRBN (1 to 442 residues)/N-terminal 6His-tagged human DDB1 (1 to 1140 residues) expressed in baculovirus infected BTI-TN-5B1-4 insect cells after 30 mins by cy5 probe based fluorescence polarization assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | BTI-TN-5B1-4 |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
From Inhibition to Degradation: Targeting the Antiapoptotic Protein Myeloid Cell Leukemia 1 (MCL1).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 3 | 6.72 | 6.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL43452 | POMALIDOMIDE | 4.0 | 1 | 6.80 |
| CHEMBL848 | LENALIDOMIDE | 4.0 | 1 | 6.75 |
| CHEMBL468 | THALIDOMIDE | 4.0 | 1 | 6.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL43452 | POMALIDOMIDE | Kd | = | 157.0 | nM | 6.80 |
| CHEMBL848 | LENALIDOMIDE | Kd | = | 178.0 | nM | 6.75 |
| CHEMBL468 | THALIDOMIDE | Kd | = | 250.0 | nM | 6.60 |