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Assay Detail

CHEMBL4398306

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of DENV2 NS2B-NS3 serine protease expressed using Benzoyl-Nle-Lys-Arg-Arg-AMC as substrate measured preincubated for 3 mins followed by substrate addition by fluorescence assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism dengue virus type 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Genome polyprotein (CHEMBL5980)
Type SINGLE PROTEIN
Organism dengue virus type 2

Publication

Recent update on anti-dengue drug discovery.
Dighe SN, Ekwudu O, Dua K, Chellappan DK, Katavic PL, Collet TA.
Eur J Med Chem (2019) 176 : 431 -455
PubMed 31128447 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.86 6.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1324 TOLCAPONE 4.0 1 6.19
CHEMBL1458891 1 5.53

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1324 TOLCAPONE IC50 = 640.0 nM 6.19
CHEMBL1458891 IC50 = 2940.0 nM 5.53