Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4398708

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine aortic smooth muscle PDE4 at 10 uM using cAMP as substrate incubated for 30 mins in presence of EGTA and [3H]cAMP by liquid scintillation counting method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design and synthesis of 3-aminophthalazine derivatives and structural analogues as PDE5 inhibitors: anti-allodynic effect against neuropathic pain in a mouse model.
Bollenbach M, Lugnier C, Kremer M, Salvat E, Megat S, Bihel F, Bourguignon JJ, Barrot M, Schmitt M.
Eur J Med Chem (2019) 177 : 269 -290
PubMed 31158744 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.43 6.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4441100 1 6.50
CHEMBL4586506 1 5.36
CHEMBL332898 1 4.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4441100 IC50 = 320.0 nM 6.50
CHEMBL4586506 IC50 = 4400.0 nM 5.36
CHEMBL332898 IC50 = 37000.0 nM 4.43