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Assay Detail

CHEMBL4399178

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST tagged VEGFR-2 expressed in baculovirus infected Sf9 cells using poly (Glu,Tyr) 4:1 as substrate in presence of ATP by Kinase-Glo luminescence assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel potent substituted 4-amino-2-thiopyrimidines as dual VEGFR-2 and BRAF kinase inhibitors.
Abdel-Mohsen HT, Omar MA, El Kerdawy AM, Mahmoud AEE, Ali MM, El Diwani HI.
Eur J Med Chem (2019) 179 : 707 -722
PubMed 31284081 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.47 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1946170 REGORAFENIB 4.0 1 8.30
CHEMBL1336 SORAFENIB 4.0 1 7.00
CHEMBL4558790 1 6.92
CHEMBL4573547 1 6.77
CHEMBL4574832 1 6.77
CHEMBL4566054 1 6.72
CHEMBL4465126 1 6.66
CHEMBL4449189 1 6.58
CHEMBL4590013 1 6.55
CHEMBL4549103 1 6.54
CHEMBL4469216 1 6.47
CHEMBL4448435 1 6.39
CHEMBL4474945 1 6.38
CHEMBL4539297 1 6.32
CHEMBL4467107 1 6.31
CHEMBL4438418 1 6.24
CHEMBL4474922 1 6.13
CHEMBL4561629 1 6.02
CHEMBL4470636 1 5.96
CHEMBL4434973 1 5.84
CHEMBL4570955 1 5.72
CHEMBL4471650 1 5.71
CHEMBL4557976 1 -
CHEMBL4453308 1 -
CHEMBL4450118 1 -
CHEMBL4547466 1 -
CHEMBL4441990 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1946170 REGORAFENIB IC50 = 5.0 nM 8.30
CHEMBL1336 SORAFENIB IC50 = 100.0 nM 7.00
CHEMBL4558790 IC50 = 120.0 nM 6.92
CHEMBL4573547 IC50 = 170.0 nM 6.77
CHEMBL4574832 IC50 = 170.0 nM 6.77
CHEMBL4566054 IC50 = 190.0 nM 6.72
CHEMBL4465126 IC50 = 220.0 nM 6.66
CHEMBL4449189 IC50 = 260.0 nM 6.58
CHEMBL4590013 IC50 = 280.0 nM 6.55
CHEMBL4549103 IC50 = 290.0 nM 6.54
CHEMBL4469216 IC50 = 340.0 nM 6.47
CHEMBL4448435 IC50 = 410.0 nM 6.39
CHEMBL4474945 IC50 = 420.0 nM 6.38
CHEMBL4539297 IC50 = 480.0 nM 6.32
CHEMBL4467107 IC50 = 490.0 nM 6.31
CHEMBL4438418 IC50 = 570.0 nM 6.24
CHEMBL4474922 IC50 = 740.0 nM 6.13
CHEMBL4561629 IC50 = 950.0 nM 6.02
CHEMBL4470636 IC50 = 1100.0 nM 5.96
CHEMBL4434973 IC50 = 1430.0 nM 5.84
CHEMBL4570955 IC50 = 1900.0 nM 5.72
CHEMBL4471650 IC50 = 1960.0 nM 5.71
CHEMBL4557976 IC50 > 10000.0 nM -
CHEMBL4453308 IC50 > 10000.0 nM -
CHEMBL4450118 IC50 > 10000.0 nM -
CHEMBL4547466 IC50 > 10000.0 nM -
CHEMBL4441990 IC50 > 10000.0 nM -