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Assay Detail

CHEMBL4400313

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged ALK (1058 to 1620 residues) cytoplasmic domain expressed in baculovirus expression system using srctide as substrate incubated for 1 hr by mobility shift assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ALK tyrosine kinase receptor (CHEMBL4247)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

An exploration of solvent-front region high affinity moiety leading to novel potent ALK & ROS1 dual inhibitors with mutant-combating effects.
Lei H, Jia F, Cao M, Wang J, Guo M, Zhu M, Zuo D, Zhai X.
Bioorg Med Chem (2019) 27 : 115051 -115051
PubMed 31492532 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 8.41 8.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4562805 1 8.68
CHEMBL4546844 1 8.60
CHEMBL2403108 CERITINIB 4.0 1 8.57
CHEMBL4462528 1 8.39
CHEMBL4536955 1 8.33
CHEMBL4448400 1 8.29
CHEMBL4471142 1 8.21
CHEMBL4468370 1 8.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4562805 IC50 = 2.1 nM 8.68
CHEMBL4546844 IC50 = 2.5 nM 8.60
CHEMBL2403108 CERITINIB IC50 = 2.7 nM 8.57
CHEMBL4462528 IC50 = 4.1 nM 8.39
CHEMBL4536955 IC50 = 4.7 nM 8.33
CHEMBL4448400 IC50 = 5.1 nM 8.29
CHEMBL4471142 IC50 = 6.2 nM 8.21
CHEMBL4468370 IC50 = 6.3 nM 8.20