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Assay Detail

CHEMBL4401535

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human (His8)2-tagged fascin (1 to 493 residues) expressed in Escherichia coli BL21(DE3) pLysS assessed as reduction in F-actin bundling preincubated for 30 mins followed by F-actin addition and measured after 45 mins by Coomassie blue staining-based SDS-PAGE analysis
27
Total Activities
27
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fascin (CHEMBL4523304)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-based design, synthesis and biological evaluation of a novel series of isoquinolone and pyrazolo[4,3-c]pyridine inhibitors of fascin 1 as potential anti-metastatic agents.
Francis S, Croft D, Schüttelkopf AW, Parry C, Pugliese A, Cameron K, Claydon S, Drysdale M, Gardner C, Gohlke A, Goodwin G, Gray CH, Konczal J, McDonald L, Mezna M, Pannifer A, Paul NR, Machesky L, McKinnon H, Bower J.
Bioorg Med Chem Lett (2019) 29 : 1023 -1029
PubMed 30773430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 5.52 6.62
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4452711 1 6.62
CHEMBL4461540 1 6.48
CHEMBL4574198 1 6.29
CHEMBL4566108 1 6.20
CHEMBL4559691 1 6.20
CHEMBL4451624 1 5.89
CHEMBL4457641 1 5.68
CHEMBL4516481 1 5.42
CHEMBL4544795 1 5.34
CHEMBL4584583 1 5.28
CHEMBL4527032 1 5.07
CHEMBL4455723 1 4.98
CHEMBL4543704 1 4.94
CHEMBL4469510 1 4.17
CHEMBL4440859 1 4.17
CHEMBL4439645 1 -
CHEMBL4564438 1 -
CHEMBL4574928 1 -
CHEMBL4537924 1 -
CHEMBL4462533 1 -
CHEMBL4472315 1 -
CHEMBL4574047 1 -
CHEMBL4448440 1 -
CHEMBL4592515 1 -
CHEMBL4576435 1 -
CHEMBL4470509 1 -
CHEMBL4539975 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4452711 IC50 = 240.0 nM 6.62
CHEMBL4461540 IC50 = 330.0 nM 6.48
CHEMBL4574198 IC50 = 510.0 nM 6.29
CHEMBL4566108 IC50 = 630.0 nM 6.20
CHEMBL4559691 IC50 = 630.0 nM 6.20
CHEMBL4451624 IC50 = 1300.0 nM 5.89
CHEMBL4457641 IC50 = 2100.0 nM 5.68
CHEMBL4516481 IC50 = 3800.0 nM 5.42
CHEMBL4544795 IC50 = 4600.0 nM 5.34
CHEMBL4584583 IC50 = 5300.0 nM 5.28
CHEMBL4527032 IC50 = 8600.0 nM 5.07
CHEMBL4455723 IC50 = 10400.0 nM 4.98
CHEMBL4543704 IC50 = 11400.0 nM 4.94
CHEMBL4469510 IC50 = 67900.0 nM 4.17
CHEMBL4440859 IC50 = 67600.0 nM 4.17
CHEMBL4439645 IC50 - -
CHEMBL4564438 IC50 - -
CHEMBL4574928 IC50 - -
CHEMBL4537924 IC50 - -
CHEMBL4462533 IC50 - -
CHEMBL4472315 IC50 - -
CHEMBL4574047 IC50 > 100000.0 nM -
CHEMBL4448440 IC50 - -
CHEMBL4592515 Inhibition - % -
CHEMBL4576435 IC50 - -
CHEMBL4470509 IC50 - -
CHEMBL4539975 IC50 - -