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Assay Detail

CHEMBL4402363

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length N-terminal GST-fused PKCi (1 to 587 residues) expressed in baculovirus expression system using 5-FAM-RFARKGSLRQKNV as substrate measured after 60 mins by caliper microfluidic mobility shift assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C iota type (CHEMBL2598)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fragment-based Discovery of a Small-Molecule Protein Kinase C-iota Inhibitor Binding Post-kinase Domain Residues.
Kwiatkowski J, Baburajendran N, Poulsen A, Liu B, Tee DHY, Wong YX, Poh ZY, Ong EH, Dinie N, Cherian J, Jansson AE, Hill J, Keller TH, Hung AW.
ACS Med Chem Lett (2019) 10 : 318 -323
PubMed 30891133 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 4.74 6.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4517782 1 6.57
CHEMBL4471416 1 6.47
CHEMBL4441139 1 6.15
CHEMBL4520505 1 4.71
CHEMBL4543879 1 4.66
CHEMBL4450200 1 4.64
CHEMBL4547613 1 4.62
CHEMBL4440509 1 4.50
CHEMBL4584982 1 4.47
CHEMBL4540971 1 4.38
CHEMBL4446447 1 4.12
CHEMBL4551875 1 4.11
CHEMBL4450468 1 3.58
CHEMBL4203838 1 3.37
CHEMBL4552099 1 -
CHEMBL4459501 1 -
CHEMBL4566521 1 -
CHEMBL4454756 1 -
CHEMBL4459301 1 -
CHEMBL4450572 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4517782 IC50 = 270.0 nM 6.57
CHEMBL4471416 IC50 = 340.0 nM 6.47
CHEMBL4441139 IC50 = 710.0 nM 6.15
CHEMBL4520505 IC50 = 19600.0 nM 4.71
CHEMBL4543879 IC50 = 22000.0 nM 4.66
CHEMBL4450200 IC50 = 23000.0 nM 4.64
CHEMBL4547613 IC50 = 24000.0 nM 4.62
CHEMBL4440509 IC50 = 32000.0 nM 4.50
CHEMBL4584982 IC50 = 34000.0 nM 4.47
CHEMBL4540971 IC50 = 42200.0 nM 4.38
CHEMBL4446447 IC50 = 76000.0 nM 4.12
CHEMBL4551875 IC50 = 77000.0 nM 4.11
CHEMBL4450468 IC50 = 263000.0 nM 3.58
CHEMBL4203838 IC50 = 424000.0 nM 3.37
CHEMBL4552099 IC50 > 384000.0 nM -
CHEMBL4459501 IC50 > 384000.0 nM -
CHEMBL4566521 IC50 > 384000.0 nM -
CHEMBL4454756 IC50 > 384000.0 nM -
CHEMBL4459301 IC50 > 384000.0 nM -
CHEMBL4450572 IC50 > 384000.0 nM -