Assay Detail
Binding
CHEMBL4402387
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 15 mins in presence of SAM and substrate followed by Ti(3+)citrate addition and measured by discontinuous HPLC analysis
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Thermus thermophilus HB8 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Aminodeoxyfutalosine synthase (CHEMBL4523327) ↗| Type | SINGLE PROTEIN |
| Organism | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) |
Publication
Antibacterial Strategy against H. pylori: Inhibition of the Radical SAM Enzyme MqnE in Menaquinone Biosynthesis.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 4.41 | 4.41 |
| Ki | 1 | 5.51 | 5.51 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4461488 | — | — | 2 | 5.51 |
| CHEMBL4534412 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4461488 | — | Ki | = | 3100.0 | nM | 5.51 |
| CHEMBL4461488 | — | IC50 | = | 38700.0 | nM | 4.41 |
| CHEMBL4534412 | — | IC50 | = | 839000.0 | nM | - |