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Assay Detail

CHEMBL4402615

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Mixed type inhibition of monophenolase activity of mushroom tyrosinase assessed as reduction in dopachrome formation using L-Tyrosine substrate by UV-Vis spectrophotometry based Dixon plot analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Agaricus bisporus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosinase (CHEMBL3318)
Type SINGLE PROTEIN
Organism Agaricus bisporus

Publication

Tyrosinase inhibitory study of flavonolignans from the seeds of Silybum marianum (Milk thistle).
Kim JY, Kim JY, Jenis J, Li ZP, Ban YJ, Baiseitova A, Park KH.
Bioorg Med Chem (2019) 27 : 2499 -2507
PubMed 30871862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 5.69 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL9509 SILIBININ 3.0 1 6.16
CHEMBL592676 ISOSILYBIN A 1 5.96
CHEMBL2397733 SILIDIANIN 2.0 1 5.77
CHEMBL130493 SILICRISTIN A 1 5.68
CHEMBL589325 SILICRISTIN B 1 5.55
CHEMBL3542340 ISOSILYBIN B 1 5.38
CHEMBL4076992 1 5.33
CHEMBL287556 KOJIC ACID 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL9509 SILIBININ Ki = 700.0 nM 6.16
CHEMBL592676 ISOSILYBIN A Ki = 1100.0 nM 5.96
CHEMBL2397733 SILIDIANIN Ki = 1700.0 nM 5.77
CHEMBL130493 SILICRISTIN A Ki = 2100.0 nM 5.68
CHEMBL589325 SILICRISTIN B Ki = 2800.0 nM 5.55
CHEMBL3542340 ISOSILYBIN B Ki = 4200.0 nM 5.38
CHEMBL4076992 Ki = 4700.0 nM 5.33
CHEMBL287556 KOJIC ACID Ki - -