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Assay Detail

CHEMBL4402619

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of diphenolase activity of mushroom tyrosinase assessed as reduction in dopachrome formation using L-DOPA substrate by UV-Vis spectrophotometry
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Agaricus bisporus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosinase (CHEMBL3318)
Type SINGLE PROTEIN
Organism Agaricus bisporus

Publication

Tyrosinase inhibitory study of flavonolignans from the seeds of Silybum marianum (Milk thistle).
Kim JY, Kim JY, Jenis J, Li ZP, Ban YJ, Baiseitova A, Park KH.
Bioorg Med Chem (2019) 27 : 2499 -2507
PubMed 30871862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.61 4.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL9509 SILIBININ 3.0 1 4.92
CHEMBL2397733 SILIDIANIN 2.0 1 4.78
CHEMBL592676 ISOSILYBIN A 1 4.78
CHEMBL3542340 ISOSILYBIN B 1 4.70
CHEMBL66 (+)-TAXIFOLIN 1 4.57
CHEMBL130493 SILICRISTIN A 1 4.54
CHEMBL4076992 1 4.45
CHEMBL287556 KOJIC ACID 1 4.43
CHEMBL589325 SILICRISTIN B 1 4.35
CHEMBL9323 DIHYDROKAEMPFEROL 1 -
CHEMBL9353 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL9509 SILIBININ IC50 = 12100.0 nM 4.92
CHEMBL2397733 SILIDIANIN IC50 = 16500.0 nM 4.78
CHEMBL592676 ISOSILYBIN A IC50 = 16700.0 nM 4.78
CHEMBL3542340 ISOSILYBIN B IC50 = 19800.0 nM 4.70
CHEMBL66 (+)-TAXIFOLIN IC50 = 27000.0 nM 4.57
CHEMBL130493 SILICRISTIN A IC50 = 28800.0 nM 4.54
CHEMBL4076992 IC50 = 35900.0 nM 4.45
CHEMBL287556 KOJIC ACID IC50 = 37100.0 nM 4.43
CHEMBL589325 SILICRISTIN B IC50 = 44900.0 nM 4.35
CHEMBL9323 DIHYDROKAEMPFEROL IC50 > 200000.0 nM -
CHEMBL9353 IC50 = 150000.0 nM -