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Assay Detail

CHEMBL4402908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human 5-LOX expressed in baculovirus-infected insect cells preincubated for 10 mins followed by Arachidonic acid and ATP addition and measured after 20 mins by H2DCFD dye based fluorescence method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL215)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

5-Lipoxygenase as a drug target: A review on trends in inhibitors structural design, SAR and mechanism based approach.
Sinha S, Doble M, Manju SL.
Bioorg Med Chem (2019) 27 : 3745 -3759
PubMed 31331653 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.64 6.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4463974 1 6.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4463974 IC50 = 229.0 nM 6.64