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Assay Detail

CHEMBL4403233

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human C-terminal His10-tagged LOXL2 (Met1 to Gln774 residues) expressed in mouse NS0 cells using cadaverine hydrochloride as substrate preincubated for 3 hrs followed by substrate addition by ROS-Glo assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysyl oxidase homolog 2 (CHEMBL3714029)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-Aminomethylene-5-sulfonylthiazole Inhibitors of Lysyl Oxidase (LOX) and LOXL2 Show Significant Efficacy in Delaying Tumor Growth.
Smithen DA, Leung LMH, Challinor M, Lawrence R, Tang H, Niculescu-Duvaz D, Pearce SP, Mcleary R, Lopes F, Aljarah M, Brown M, Johnson L, Thomson G, Marais R, Springer C.
J Med Chem (2020) 63 : 2308 -2324
PubMed 31430136 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.74 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4568508 1 7.16
CHEMBL4457897 1 7.16
CHEMBL4444163 1 7.11
CHEMBL4550042 1 7.07
CHEMBL4457629 1 6.92
CHEMBL4441173 1 6.90
CHEMBL4574361 1 6.84
CHEMBL4463882 1 6.75
CHEMBL1618272 1 6.43
CHEMBL4443558 1 6.33
CHEMBL4565942 1 5.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4568508 IC50 = 69.0 nM 7.16
CHEMBL4457897 IC50 = 69.0 nM 7.16
CHEMBL4444163 IC50 = 78.0 nM 7.11
CHEMBL4550042 IC50 = 85.0 nM 7.07
CHEMBL4457629 IC50 = 120.0 nM 6.92
CHEMBL4441173 IC50 = 126.0 nM 6.90
CHEMBL4574361 IC50 = 146.0 nM 6.84
CHEMBL4463882 IC50 = 176.0 nM 6.75
CHEMBL1618272 IC50 = 372.0 nM 6.43
CHEMBL4443558 IC50 = 470.0 nM 6.33
CHEMBL4565942 IC50 = 3190.0 nM 5.50