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Assay Detail

CHEMBL4404615

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Allosteric inhibition of [3H]-2-tert-butoxy-2-[2-methyl-4-(p-tolyl)-3-quinolyl]acetic acid binding to HIV1 integrase measured after 1 hr under dark condition by microbeta plate reader method
18
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 integrase (CHEMBL3471)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Discovery and Optimization of Novel Pyrazolopyrimidines as Potent and Orally Bioavailable Allosteric HIV-1 Integrase Inhibitors.
Li G, Meanwell NA, Krystal MR, Langley DR, Naidu BN, Sivaprakasam P, Lewis H, Kish K, Khan JA, Ng A, Trainor GL, Cianci C, Dicker IB, Walker MA, Lin Z, Protack T, Discotto L, Jenkins S, Gerritz SW, Pendri A.
J Med Chem (2020) 63 : 2620 -2637
PubMed 32081010 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 5.99 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4476366 1 7.40
CHEMBL4460705 1 7.22
CHEMBL4454255 1 7.05
CHEMBL4573064 2 6.77
CHEMBL4565963 1 6.51
CHEMBL4538853 1 6.37
CHEMBL4461955 1 6.17
CHEMBL4469948 1 5.70
CHEMBL4541973 1 5.47
CHEMBL4452801 1 5.43
CHEMBL4475105 1 5.36
CHEMBL4587557 1 4.82
CHEMBL4560817 1 4.17
CHEMBL4440179 1 -
CHEMBL4592796 1 -
CHEMBL4551451 1 -
CHEMBL4439901 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4476366 Ki = 40.0 nM 7.40
CHEMBL4460705 Ki = 60.0 nM 7.22
CHEMBL4454255 Ki = 90.0 nM 7.05
CHEMBL4573064 Ki = 170.0 nM 6.77
CHEMBL4565963 Ki = 310.0 nM 6.51
CHEMBL4538853 Ki = 430.0 nM 6.37
CHEMBL4461955 Ki = 680.0 nM 6.17
CHEMBL4469948 Ki = 2000.0 nM 5.70
CHEMBL4541973 Ki = 3400.0 nM 5.47
CHEMBL4573064 Ki = 3400.0 nM 5.47
CHEMBL4452801 Ki = 3700.0 nM 5.43
CHEMBL4475105 Ki = 4400.0 nM 5.36
CHEMBL4587557 Ki = 15300.0 nM 4.82
CHEMBL4560817 Ki = 67000.0 nM 4.17
CHEMBL4440179 Ki > 40000.0 nM -
CHEMBL4592796 Ki > 40000.0 nM -
CHEMBL4551451 Ki > 40000.0 nM -
CHEMBL4439901 Ki > 53000.0 nM -