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Assay Detail

CHEMBL4404704

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDE4D2 (86 to 413 residues) expressed in Escherichia coli BL21 codon-plus cells using [3H]cAMP as substrate incubated for 15 mins by liquid scintillation counting method
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4D (CHEMBL288)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Optimization of α-Mangostin Derivatives as Novel PDE4 Inhibitors for the Treatment of Vascular Dementia.
Liang J, Huang YY, Zhou Q, Gao Y, Li Z, Wu D, Yu S, Guo L, Chen Z, Huang L, Liang SH, He X, Wu R, Luo HB.
J Med Chem (2020) 63 : 3370 -3380
PubMed 32115956 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.85 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4453774 1 7.80
CHEMBL4471352 1 7.77
CHEMBL4529685 1 7.36
CHEMBL4435746 1 7.24
CHEMBL4473291 1 7.12
CHEMBL4515248 1 7.00
CHEMBL4567905 1 7.00
CHEMBL4536071 1 6.83
CHEMBL4563050 1 6.70
CHEMBL561643 MANGOSTANIN 1 6.38
CHEMBL4434646 1 6.32
CHEMBL63 ROLIPRAM 2.0 1 6.27
CHEMBL4524406 1 6.21
CHEMBL323197 MANGOSTIN 1 5.88
CHEMBL4468003 1 -
CHEMBL4440868 1 -
CHEMBL4456328 1 -
CHEMBL4468793 1 -
CHEMBL4463249 1 -
CHEMBL4450759 1 -
CHEMBL4474532 1 -
CHEMBL4533012 1 -
CHEMBL4474598 1 -
CHEMBL4514622 1 -
CHEMBL4545698 1 -
CHEMBL4436788 1 -
CHEMBL4551152 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4453774 IC50 = 16.0 nM 7.80
CHEMBL4471352 IC50 = 17.0 nM 7.77
CHEMBL4529685 IC50 = 44.0 nM 7.36
CHEMBL4435746 IC50 = 58.0 nM 7.24
CHEMBL4473291 IC50 = 76.0 nM 7.12
CHEMBL4515248 IC50 = 99.0 nM 7.00
CHEMBL4567905 IC50 = 99.0 nM 7.00
CHEMBL4536071 IC50 = 148.0 nM 6.83
CHEMBL4563050 IC50 = 200.0 nM 6.70
CHEMBL561643 MANGOSTANIN IC50 = 420.0 nM 6.38
CHEMBL4434646 IC50 = 480.0 nM 6.32
CHEMBL63 ROLIPRAM IC50 = 540.0 nM 6.27
CHEMBL4524406 IC50 = 622.0 nM 6.21
CHEMBL323197 MANGOSTIN IC50 = 1310.0 nM 5.88
CHEMBL4468003 IC50 > 10000.0 nM -
CHEMBL4440868 IC50 > 10000.0 nM -
CHEMBL4456328 IC50 > 10000.0 nM -
CHEMBL4468793 IC50 > 10000.0 nM -
CHEMBL4463249 IC50 > 10000.0 nM -
CHEMBL4450759 IC50 > 10000.0 nM -
CHEMBL4474532 IC50 > 10000.0 nM -
CHEMBL4533012 IC50 > 10000.0 nM -
CHEMBL4474598 IC50 > 10000.0 nM -
CHEMBL4514622 IC50 > 1000.0 nM -
CHEMBL4545698 IC50 > 10000.0 nM -
CHEMBL4436788 IC50 > 10000.0 nM -
CHEMBL4551152 IC50 > 10000.0 nM -