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Assay Detail

CHEMBL4404829

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK2/JAK2 in human PBMC assessed as reduction in GM-CSF-induced STAT5 phosphorylation pre-incubated for 30 mins before GM-CSF stimulation for 30 mins AlphaLISAassay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Gut-Restricted JAK Inhibitor for the Treatment of Inflammatory Bowel Disease.
Leonard KA, Madge LA, Krawczuk PJ, Wang A, Kreutter KD, Bacani GM, Chai W, Smith RC, Tichenor MS, Harris MC, Malaviya R, Seierstad M, Johnson ME, Venable JD, Kim S, Hirst GC, Mathur AS, Rao TS, Edwards JP, Rizzolio MC, Koudriakova T.
J Med Chem (2020) 63 : 2915 -2929
PubMed 32134643 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.92 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4588333 1 7.55
CHEMBL221959 TOFACITINIB 4.0 1 7.42
CHEMBL4439418 1 7.41
CHEMBL4537718 1 7.16
CHEMBL4473572 1 7.08
CHEMBL4563534 1 7.05
CHEMBL4437638 1 6.90
CHEMBL4514898 1 6.83
CHEMBL4456630 1 6.61
CHEMBL4582390 1 6.40
CHEMBL4447497 1 5.71
CHEMBL4470339 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4588333 IC50 = 28.0 nM 7.55
CHEMBL221959 TOFACITINIB IC50 = 38.0 nM 7.42
CHEMBL4439418 IC50 = 39.0 nM 7.41
CHEMBL4537718 IC50 = 69.0 nM 7.16
CHEMBL4473572 IC50 = 83.0 nM 7.08
CHEMBL4563534 IC50 = 90.0 nM 7.05
CHEMBL4437638 IC50 = 126.0 nM 6.90
CHEMBL4514898 IC50 = 148.0 nM 6.83
CHEMBL4456630 IC50 = 243.0 nM 6.61
CHEMBL4582390 IC50 = 399.0 nM 6.40
CHEMBL4447497 IC50 = 1962.0 nM 5.71
CHEMBL4470339 IC50 - -