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Assay Detail

CHEMBL4406077

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human 5HT2C receptor stably expressed in HEK293 cells co-expressing Gq assessed as inhibition of 5-HT-induced calcium mobilization preincubated for 15 mins followed by 5-HT addition and measured at 1 sec interval for 60 secs by Fluo-4 calcium dye based FLIPR assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2C (CHEMBL225)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Bitopic 2-Phenylcyclopropylmethylamine (PCPMA) Derivatives as Selective Dopamine D3 Receptor Ligands.
Tan L, Zhou Q, Yan W, Sun J, Kozikowski AP, Zhao S, Huang XP, Cheng J.
J Med Chem (2020) 63 : 4579 -4602
PubMed 32282200 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.18 6.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4593394 1 6.07
CHEMBL4443039 1 5.00
CHEMBL4475406 1 4.84
CHEMBL4454380 1 4.79
CHEMBL4439544 1 -
CHEMBL240773 QUINPIROLE 2.0 1 -
CHEMBL59 DOPAMINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4593394 IC50 = 860.0 nM 6.07
CHEMBL4443039 IC50 = 10100.0 nM 5.00
CHEMBL4475406 IC50 = 14500.0 nM 4.84
CHEMBL4454380 IC50 = 16100.0 nM 4.79
CHEMBL4439544 IC50 > 30000.0 nM -
CHEMBL240773 QUINPIROLE IC50 - -
CHEMBL59 DOPAMINE IC50 - -