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Assay Detail

CHEMBL4406242

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal GST-tagged JAK1 (866 to 1154 residues) expressed in insect cells using FITC-labeled C6-KKHTDDGYMPMSPGVA-NH peptide as substrate after 10 mins in presence of 5 mM ATP by caliper mobility shift assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (2R)-N-[3-[2-[(3-Methoxy-1-methyl-pyrazol-4-yl)amino]pyrimidin-4-yl]-1H-indol-7-yl]-2-(4-methylpiperazin-1-yl)propenamide (AZD4205) as a Potent and Selective Janus Kinase 1 Inhibitor.
Su Q, Banks E, Bebernitz G, Bell K, Borenstein CF, Chen H, Chuaqui CE, Deng N, Ferguson AD, Kawatkar S, Grimster NP, Ruston L, Lyne PD, Read JA, Peng X, Pei X, Fawell S, Tang Z, Throner S, Vasbinder MM, Wang H, Winter-Holt J, Woessner R, Wu A, Yang W, Zinda M, Kettle JG.
J Med Chem (2020) 63 : 4517 -4527
PubMed 32297743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.13 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4446962 1 8.40
CHEMBL4064366 1 8.15
CHEMBL4577393 1 8.10
CHEMBL4450982 1 8.00
CHEMBL4441479 1 7.75
CHEMBL4541637 1 7.72
CHEMBL4544007 1 7.70
CHEMBL1789941 RUXOLITINIB 4.0 1 7.70
CHEMBL4466590 1 7.57
CHEMBL4536860 1 7.40
CHEMBL4452410 1 7.37
CHEMBL4577523 GOLIDOCITINIB 2.0 1 7.16
CHEMBL4444782 1 7.16
CHEMBL4449567 1 6.89
CHEMBL4588587 1 6.75
CHEMBL4466247 1 6.58
CHEMBL4553035 1 6.34
CHEMBL4551327 1 5.96
CHEMBL4588128 1 5.77
CHEMBL4517840 1 5.66
CHEMBL4476801 1 5.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4446962 IC50 = 4.0 nM 8.40
CHEMBL4064366 IC50 = 7.0 nM 8.15
CHEMBL4577393 IC50 = 8.0 nM 8.10
CHEMBL4450982 IC50 = 10.0 nM 8.00
CHEMBL4441479 IC50 = 18.0 nM 7.75
CHEMBL4541637 IC50 = 19.0 nM 7.72
CHEMBL4544007 IC50 = 20.0 nM 7.70
CHEMBL1789941 RUXOLITINIB IC50 = 20.0 nM 7.70
CHEMBL4466590 IC50 = 27.0 nM 7.57
CHEMBL4536860 IC50 = 40.0 nM 7.40
CHEMBL4452410 IC50 = 43.0 nM 7.37
CHEMBL4577523 GOLIDOCITINIB IC50 = 70.0 nM 7.16
CHEMBL4444782 IC50 = 70.0 nM 7.16
CHEMBL4449567 IC50 = 130.0 nM 6.89
CHEMBL4588587 IC50 = 180.0 nM 6.75
CHEMBL4466247 IC50 = 260.0 nM 6.58
CHEMBL4553035 IC50 = 460.0 nM 6.34
CHEMBL4551327 IC50 = 1100.0 nM 5.96
CHEMBL4588128 IC50 = 1700.0 nM 5.77
CHEMBL4517840 IC50 = 2200.0 nM 5.66
CHEMBL4476801 IC50 = 2400.0 nM 5.62