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Assay Detail

CHEMBL4406248

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Binding
Inhibition of JAK1 in human NCI-H1975 cells assessed as decrease in STAT3 phosphorylation measured after 1 hr by ELISA
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type NCI-H1975
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (2R)-N-[3-[2-[(3-Methoxy-1-methyl-pyrazol-4-yl)amino]pyrimidin-4-yl]-1H-indol-7-yl]-2-(4-methylpiperazin-1-yl)propenamide (AZD4205) as a Potent and Selective Janus Kinase 1 Inhibitor.
Su Q, Banks E, Bebernitz G, Bell K, Borenstein CF, Chen H, Chuaqui CE, Deng N, Ferguson AD, Kawatkar S, Grimster NP, Ruston L, Lyne PD, Read JA, Peng X, Pei X, Fawell S, Tang Z, Throner S, Vasbinder MM, Wang H, Winter-Holt J, Woessner R, Wu A, Yang W, Zinda M, Kettle JG.
J Med Chem (2020) 63 : 4517 -4527
PubMed 32297743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.97 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4064366 1 7.22
CHEMBL4466590 1 7.10
CHEMBL4577393 1 7.03
CHEMBL4541637 1 6.92
CHEMBL4450982 1 6.89
CHEMBL4577523 GOLIDOCITINIB 2.0 1 6.89
CHEMBL4449567 1 6.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4064366 IC50 = 60.0 nM 7.22
CHEMBL4466590 IC50 = 80.0 nM 7.10
CHEMBL4577393 IC50 = 94.0 nM 7.03
CHEMBL4541637 IC50 = 120.0 nM 6.92
CHEMBL4450982 IC50 = 130.0 nM 6.89
CHEMBL4577523 GOLIDOCITINIB IC50 = 130.0 nM 6.89
CHEMBL4449567 IC50 = 180.0 nM 6.75