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Assay Detail

CHEMBL4408646

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant human TRPV1 expressed in CHO cells assessed as as inhibition of capsaicin-induced calcium uptake incubated for 5 mins in presence of 45Ca2+ by liquid scintillation counter
18
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of dual-acting opioid ligand and TRPV1 antagonists as novel therapeutic agents for pain.
Lee H, Ahn S, Ann J, Ha H, Yoo YD, Kim YH, Hwang JY, Hur KH, Jang CG, Pearce LV, Esch TE, Lewin NE, Blumberg PM, Lee J.
Eur J Med Chem (2019) 182 : 111634 -111634
PubMed 31472474 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 7.58 8.65
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2177429 1 8.65
CHEMBL4586277 1 8.09
CHEMBL4553702 1 8.06
CHEMBL4471708 1 8.05
CHEMBL4438643 1 8.00
CHEMBL4442473 1 7.73
CHEMBL4571845 1 7.60
CHEMBL4562769 1 7.51
CHEMBL4457595 1 7.50
CHEMBL4438974 1 7.50
CHEMBL4459611 1 7.38
CHEMBL4453980 1 7.25
CHEMBL4555508 1 7.09
CHEMBL4476530 1 7.07
CHEMBL4536869 1 6.92
CHEMBL4457304 1 6.81
CHEMBL4483060 1 -
CHEMBL4448866 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2177429 EC50 = 2.22 nM 8.65
CHEMBL4586277 EC50 = 8.2 nM 8.09
CHEMBL4553702 EC50 = 8.8 nM 8.06
CHEMBL4471708 EC50 = 8.9 nM 8.05
CHEMBL4438643 EC50 = 10.1 nM 8.00
CHEMBL4442473 EC50 = 18.68 nM 7.73
CHEMBL4571845 EC50 = 25.0 nM 7.60
CHEMBL4562769 EC50 = 30.6 nM 7.51
CHEMBL4457595 EC50 = 31.3 nM 7.50
CHEMBL4438974 EC50 = 32.0 nM 7.50
CHEMBL4459611 EC50 = 42.0 nM 7.38
CHEMBL4453980 EC50 = 56.1 nM 7.25
CHEMBL4555508 EC50 = 81.0 nM 7.09
CHEMBL4476530 EC50 = 85.9 nM 7.07
CHEMBL4536869 EC50 = 120.0 nM 6.92
CHEMBL4457304 EC50 = 156.0 nM 6.81
CHEMBL4483060 Inhibition - % -
CHEMBL4448866 EC50 - -