Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4409429

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human C-terminal His8-tagged IDH1 R132H mutant expressed in Escherichia coli BL21 assessed as reduction in NADPH consumption using alpha-ketoglutarate as substrate preincubated for 60 mins followed by substrate addition measured for 60 mins in presence of NADPH by diaphorase/resazurin-based assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of new small molecule inhibitors targeting isocitrate dehydrogenase 1 (IDH1) with blood-brain barrier penetration.
Cao H, Zhu G, Sun L, Chen G, Ma X, Luo X, Zhu J.
Eur J Med Chem (2019) 183 : 111694 -111694
PubMed 31561044 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.76 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4444067 1 9.00
CHEMBL4529476 1 8.68
CHEMBL4548637 1 8.67
CHEMBL4469477 1 8.66
CHEMBL4467991 1 8.64
CHEMBL4475715 1 8.57
CHEMBL3989958 IVOSIDENIB 4.0 1 8.46
CHEMBL4568616 1 8.40
CHEMBL4451801 1 7.81
CHEMBL4474186 1 7.74
CHEMBL4476025 1 7.72
CHEMBL4553096 1 7.62
CHEMBL4455559 1 7.62
CHEMBL4445771 1 7.48
CHEMBL4567884 1 7.47
CHEMBL4570265 1 7.38
CHEMBL4537907 1 7.37
CHEMBL4592898 1 7.35
CHEMBL4546041 1 7.32
CHEMBL3947537 1 7.30
CHEMBL4541801 1 7.29
CHEMBL4470532 1 7.21
CHEMBL4455018 1 6.92
CHEMBL4532585 1 6.89
CHEMBL4575435 1 6.32
CHEMBL4474717 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4444067 IC50 = 1.0 nM 9.00
CHEMBL4529476 IC50 = 2.1 nM 8.68
CHEMBL4548637 IC50 = 2.12 nM 8.67
CHEMBL4469477 IC50 = 2.2 nM 8.66
CHEMBL4467991 IC50 = 2.3 nM 8.64
CHEMBL4475715 IC50 = 2.7 nM 8.57
CHEMBL3989958 IVOSIDENIB IC50 = 3.5 nM 8.46
CHEMBL4568616 IC50 = 4.0 nM 8.40
CHEMBL4451801 IC50 = 15.5 nM 7.81
CHEMBL4474186 IC50 = 18.4 nM 7.74
CHEMBL4476025 IC50 = 19.2 nM 7.72
CHEMBL4553096 IC50 = 24.1 nM 7.62
CHEMBL4455559 IC50 = 23.8 nM 7.62
CHEMBL4445771 IC50 = 33.4 nM 7.48
CHEMBL4567884 IC50 = 33.8 nM 7.47
CHEMBL4570265 IC50 = 42.0 nM 7.38
CHEMBL4537907 IC50 = 42.7 nM 7.37
CHEMBL4592898 IC50 = 44.3 nM 7.35
CHEMBL4546041 IC50 = 47.8 nM 7.32
CHEMBL3947537 IC50 = 49.6 nM 7.30
CHEMBL4541801 IC50 = 50.8 nM 7.29
CHEMBL4470532 IC50 = 61.9 nM 7.21
CHEMBL4455018 IC50 = 119.8 nM 6.92
CHEMBL4532585 IC50 = 128.5 nM 6.89
CHEMBL4575435 IC50 = 478.5 nM 6.32
CHEMBL4474717 IC50 > 10000.0 nM -