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Assay Detail

CHEMBL4409780

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at recombinant human GPR119 expressed in HEK293 cells after 24 hrs by steady-glo luciferase assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucose-dependent insulinotropic receptor (CHEMBL5652)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

One-pot synthesis of novel tert-butyl-4-substituted phenyl-1H-1,2,3-triazolo piperazine/piperidine carboxylates, potential GPR119 agonists.
Bashetti N, Shanmukha Kumar JV, Seelam NV, Prasanna B, Mintz A, Damuka N, Devanathan S, Solingapuram Sai KK.
Bioorg Med Chem Lett (2019) 29 : 126707 -126707
PubMed 31630858 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 8.12 8.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4439483 1 8.51
CHEMBL4578862 1 8.45
CHEMBL4475130 1 8.44
CHEMBL4435207 1 8.38
CHEMBL461384 1 8.32
CHEMBL4457078 1 8.15
CHEMBL4521163 1 8.09
CHEMBL4445174 1 8.06
CHEMBL4591642 1 7.98
CHEMBL4473081 1 7.95
CHEMBL4456666 1 7.91
CHEMBL4461803 1 7.91
CHEMBL4461083 1 7.91
CHEMBL4464713 1 7.87
CHEMBL4454792 1 7.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4439483 EC50 = 3.12 nM 8.51
CHEMBL4578862 EC50 = 3.59 nM 8.45
CHEMBL4475130 EC50 = 3.67 nM 8.44
CHEMBL4435207 EC50 = 4.21 nM 8.38
CHEMBL461384 EC50 = 4.78 nM 8.32
CHEMBL4457078 EC50 = 7.12 nM 8.15
CHEMBL4521163 EC50 = 8.17 nM 8.09
CHEMBL4445174 EC50 = 8.78 nM 8.06
CHEMBL4591642 EC50 = 10.43 nM 7.98
CHEMBL4473081 EC50 = 11.12 nM 7.95
CHEMBL4456666 EC50 = 12.34 nM 7.91
CHEMBL4461803 EC50 = 12.43 nM 7.91
CHEMBL4461083 EC50 = 12.31 nM 7.91
CHEMBL4464713 EC50 = 13.56 nM 7.87
CHEMBL4454792 EC50 = 13.98 nM 7.85