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Assay Detail

CHEMBL4410607

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of Plasmodium falciparum dihydrofolate reductase N51I/C59R/S108N/I164L quadruple mutant expressed in Escherichia coli BL21(DE3)pLysS using dihydrofolate as substrate in presence of NADPH by UV-vis spectrophotometry analysis
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

6-Hydrophobic aromatic substituent pyrimethamine analogues as potential antimalarials for pyrimethamine-resistant Plasmodium falciparum.
Saepua S, Sadorn K, Vanichtanankul J, Anukunwithaya T, Rattanajak R, Vitsupakorn D, Kamchonwongpaisan S, Yuthavong Y, Thongpanchang C.
Bioorg Med Chem (2019) 27 : 115158 -115158
PubMed 31685330 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 27 8.40 9.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4590684 1 9.39
CHEMBL4452609 1 9.30
CHEMBL4447272 1 9.14
CHEMBL4462327 1 9.03
CHEMBL4461699 1 9.01
CHEMBL4588852 1 8.98
CHEMBL4565928 1 8.94
CHEMBL4546946 1 8.91
CHEMBL4586252 1 8.90
CHEMBL4553483 1 8.86
CHEMBL4558959 1 8.83
CHEMBL4526752 1 8.76
CHEMBL4459902 1 8.51
CHEMBL4437388 1 8.49
CHEMBL278847 1 8.48
CHEMBL4467953 1 8.44
CHEMBL4566524 1 8.30
CHEMBL4468791 1 8.30
CHEMBL4444614 1 8.12
CHEMBL4515405 1 8.06
CHEMBL4456065 1 7.80
CHEMBL4441455 1 7.77
CHEMBL4557136 1 7.65
CHEMBL4459356 1 7.56
CHEMBL22148 1 7.50
CHEMBL4542001 1 7.48
CHEMBL36 PYRIMETHAMINE 4.0 1 6.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4590684 Ki = 0.41 nM 9.39
CHEMBL4452609 Ki = 0.5 nM 9.30
CHEMBL4447272 Ki = 0.72 nM 9.14
CHEMBL4462327 Ki = 0.93 nM 9.03
CHEMBL4461699 Ki = 0.98 nM 9.01
CHEMBL4588852 Ki = 1.04 nM 8.98
CHEMBL4565928 Ki = 1.16 nM 8.94
CHEMBL4546946 Ki = 1.24 nM 8.91
CHEMBL4586252 Ki = 1.26 nM 8.90
CHEMBL4553483 Ki = 1.37 nM 8.86
CHEMBL4558959 Ki = 1.49 nM 8.83
CHEMBL4526752 Ki = 1.73 nM 8.76
CHEMBL4459902 Ki = 3.06 nM 8.51
CHEMBL4437388 Ki = 3.2 nM 8.49
CHEMBL278847 Ki = 3.3 nM 8.48
CHEMBL4467953 Ki = 3.61 nM 8.44
CHEMBL4566524 Ki = 5.04 nM 8.30
CHEMBL4468791 Ki = 4.96 nM 8.30
CHEMBL4444614 Ki = 7.5 nM 8.12
CHEMBL4515405 Ki = 8.71 nM 8.06
CHEMBL4456065 Ki = 15.89 nM 7.80
CHEMBL4441455 Ki = 17.08 nM 7.77
CHEMBL4557136 Ki = 22.44 nM 7.65
CHEMBL4459356 Ki = 27.48 nM 7.56
CHEMBL22148 Ki = 31.9 nM 7.50
CHEMBL4542001 Ki = 33.32 nM 7.48
CHEMBL36 PYRIMETHAMINE Ki = 385.0 nM 6.42