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Assay Detail

CHEMBL4410839

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length recombinant human NAMPT using N15-NAM as substrate preincubated for 15 mins followed by substrate addition and measured after 90 mins by LCMS analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide phosphoribosyltransferase (CHEMBL1744525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Scaffold Morphing Identifies 3-Pyridyl Azetidine Ureas as Inhibitors of Nicotinamide Phosphoribosyltransferase (NAMPT).
Palacios DS, Meredith EL, Kawanami T, Adams CM, Chen X, Darsigny V, Palermo M, Baird D, George EL, Guy C, Hewett J, Tierney L, Thigale S, Wang L, Weihofen WA.
ACS Med Chem Lett (2019) 10 : 1524 -1529
PubMed 31749905 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.57 8.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4546918 1 8.57
CHEMBL4205098 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4546918 IC50 = 2.7 nM 8.57
CHEMBL4205098 IC50 < 1.0 nM -