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Assay Detail

CHEMBL4410949

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant GST-tagged mTOR catalytic domain (1360 to 2549 residues) expressed in baculovirus expression system using GFP-4E-BP1 peptide as substrate incubated for 1 hr by LanthaScreen assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Impact of Minor Structural Modifications on Properties of a Series of mTOR Inhibitors.
Ouvry G, Clary L, Tomas L, Aurelly M, Bonnary L, Borde E, Bouix-Peter C, Chantalat L, Defoin-Platel C, Deret S, Forissier M, Harris CS, Isabet T, Lamy L, Luzy AP, Pascau J, Soulet C, Taddei A, Taquet N, Thoreau E, Varvier E, Vial E, Hennequin LF.
ACS Med Chem Lett (2019) 10 : 1561 -1567
PubMed 31749911 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 7.23 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3545097 SAPANISERTIB 2.0 1 9.00
CHEMBL4529672 1 8.40
CHEMBL4564479 1 8.22
CHEMBL4549761 1 8.00
CHEMBL4572049 1 7.96
CHEMBL4538004 1 7.89
CHEMBL4441763 1 7.70
CHEMBL4588610 1 7.68
CHEMBL4585005 1 7.55
CHEMBL4538629 1 7.48
CHEMBL4517253 1 7.40
CHEMBL4461815 1 7.30
CHEMBL4475845 1 7.21
CHEMBL4456417 1 6.72
CHEMBL4525459 1 6.43
CHEMBL4451788 1 5.77
CHEMBL4440554 1 5.66
CHEMBL4475002 1 5.64
CHEMBL4522109 1 5.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3545097 SAPANISERTIB Ki = 1.0 nM 9.00
CHEMBL4529672 Ki = 4.0 nM 8.40
CHEMBL4564479 Ki = 6.0 nM 8.22
CHEMBL4549761 Ki = 10.0 nM 8.00
CHEMBL4572049 Ki = 11.0 nM 7.96
CHEMBL4538004 Ki = 13.0 nM 7.89
CHEMBL4441763 Ki = 20.0 nM 7.70
CHEMBL4588610 Ki = 21.0 nM 7.68
CHEMBL4585005 Ki = 28.0 nM 7.55
CHEMBL4538629 Ki = 33.0 nM 7.48
CHEMBL4517253 Ki = 40.0 nM 7.40
CHEMBL4461815 Ki = 50.0 nM 7.30
CHEMBL4475845 Ki = 62.0 nM 7.21
CHEMBL4456417 Ki = 190.0 nM 6.72
CHEMBL4525459 Ki = 370.0 nM 6.43
CHEMBL4451788 Ki = 1700.0 nM 5.77
CHEMBL4440554 Ki = 2200.0 nM 5.66
CHEMBL4475002 Ki = 2300.0 nM 5.64
CHEMBL4522109 Ki = 4000.0 nM 5.40