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Assay Detail

CHEMBL4413404

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat FLAG-tagged DGKalpha expressed in HEK293T cells using DAG as substrate measured after 15 mins in presence of [gamma-32P]ATP by scintillation counting method
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Diacylglycerol kinase alpha (CHEMBL4523237)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Identification of a novel DGKα inhibitor for XLP-1 therapy by virtual screening.
Velnati S, Ruffo E, Massarotti A, Talmon M, Varma KSS, Gesu A, Fresu LG, Snow AL, Bertoni A, Capello D, Tron GC, Graziani A, Baldanzi G.
Eur J Med Chem (2019) 164 : 378 -390
PubMed 30611057 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.70 4.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL259388 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL259388 IC50 = 20000.0 nM 4.70