Assay Detail
Binding
CHEMBL4413706
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NS5B polymerase in HCV genotype 2a infected in human HuH7 replicon cells after 5 days by RT-PCR analysis
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus subtype 2a |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
RNA-directed RNA polymerase (CHEMBL4296320) ↗| Type | SINGLE PROTEIN |
| Organism | Hepacivirus hominis |
Publication
Discovery of a Series of 2'-α-Fluoro,2'-β-bromo-ribonucleosides and Their Phosphoramidate Prodrugs as Potent Pan-Genotypic Inhibitors of Hepatitis C Virus.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 7.17 | 7.37 |
| EC90 | 3 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1259059 | SOFOSBUVIR | 4.0 | 2 | 7.37 |
| CHEMBL4455349 | — | — | 2 | 7.34 |
| CHEMBL4469773 | — | — | 2 | 6.80 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1259059 | SOFOSBUVIR | EC50 | = | 43.0 | nM | 7.37 |
| CHEMBL4455349 | — | EC50 | = | 46.0 | nM | 7.34 |
| CHEMBL4469773 | — | EC50 | = | 160.0 | nM | 6.80 |
| CHEMBL4469773 | — | EC90 | = | 0.83 | uM | - |
| CHEMBL4455349 | — | EC90 | = | 0.077 | uM | - |
| CHEMBL1259059 | SOFOSBUVIR | EC90 | = | 0.13 | uM | - |