Assay Detail
Binding
CHEMBL4413714
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NS5B polymerase in HCV genotype 1b/5a chimeric replicon infected in human HuH7 replicon cells after 5 days by RT-PCR analysis
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
RNA-directed RNA polymerase (CHEMBL4296320) ↗| Type | SINGLE PROTEIN |
| Organism | Hepacivirus hominis |
Publication
Discovery of a Series of 2'-α-Fluoro,2'-β-bromo-ribonucleosides and Their Phosphoramidate Prodrugs as Potent Pan-Genotypic Inhibitors of Hepatitis C Virus.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 7.19 | 7.52 |
| EC90 | 3 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4455349 | — | — | 2 | 7.52 |
| CHEMBL1259059 | SOFOSBUVIR | 4.0 | 2 | 7.36 |
| CHEMBL4469773 | — | — | 2 | 6.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4455349 | — | EC50 | = | 30.0 | nM | 7.52 |
| CHEMBL1259059 | SOFOSBUVIR | EC50 | = | 44.0 | nM | 7.36 |
| CHEMBL4469773 | — | EC50 | = | 210.0 | nM | 6.68 |
| CHEMBL4469773 | — | EC90 | = | 0.8 | uM | - |
| CHEMBL4455349 | — | EC90 | = | 0.083 | uM | - |
| CHEMBL1259059 | SOFOSBUVIR | EC90 | = | 0.32 | uM | - |