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Assay Detail

CHEMBL4413728

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NS5B polymerase in HCV genotype 4a infected in human HuH7 replicon cells after 96 hrs by RT-PCR analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 4a
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

RNA-directed RNA polymerase (CHEMBL4296320)
Type SINGLE PROTEIN
Organism Hepacivirus hominis

Publication

Discovery of a Series of 2'-α-Fluoro,2'-β-bromo-ribonucleosides and Their Phosphoramidate Prodrugs as Potent Pan-Genotypic Inhibitors of Hepatitis C Virus.
Mengshetti S, Zhou L, Sari O, De Schutter C, Zhang H, Cho JH, Tao S, Bassit LC, Verma K, Domaoal RA, Ehteshami M, Jiang Y, Ovadia R, Kasthuri M, Ollinger Russell O, McBrayer T, Whitaker T, Pattassery J, Pascual ML, Uher L, Lin BY, Lee S, Amblard F, Coats SJ, Schinazi RF.
J Med Chem (2019) 62 : 1859 -1874
PubMed 30653317 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.70 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL521487 1 7.16
CHEMBL486231 GS-461203 -1.0 1 6.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL521487 IC50 = 70.0 nM 7.16
CHEMBL486231 GS-461203 IC50 = 570.0 nM 6.24