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Assay Detail

CHEMBL4415206

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Electrophorus electricus AChE using acetylthiocholine iodide as substrate preincubated for 30 mins followed by substrate addition and measured after 5 mins by Ellman's method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

Labdanes, Withanolides, and Other Constituents from <i>Physalis nicandroides</i>.
Torres FR, Pérez-Castorena AL, Arredondo L, Toscano RA, Nieto-Camacho A, Martínez M, Maldonado E.
J Nat Prod (2019) 82 : 2489 -2500
PubMed 31429569 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.36 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL94 PHYSOSTIGMINE 3.0 1 8.52
CHEMBL4577484 1 4.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL94 PHYSOSTIGMINE IC50 = 3.0 nM 8.52
CHEMBL4577484 IC50 = 64400.0 nM 4.19