Assay Detail
Binding
CHEMBL4417324
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant 5-LOX expressed in insect cells assessed as decrease in production of 5-HPETE and 5-HETE using arachidonic acid as substrate preincubated for 5 mins followed by substrate addition measured after 20 mins in dark by ferric ion oxidation-xylenol orange method
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL215) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-Activity Relationships of Pentacyclic Triterpenoids as Inhibitors of Cyclooxygenase and Lipoxygenase Enzymes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 30 | 4.49 | 4.62 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL93 | ZILEUTON | 4.0 | 1 | 4.62 |
| CHEMBL230006 | ENOXOLONE | 2.0 | 1 | 4.61 |
| CHEMBL4561483 | — | — | 1 | 4.59 |
| CHEMBL437964 | 11-KETO BOSWELLIC ACID | — | 1 | 4.55 |
| CHEMBL520726 | — | — | 1 | 4.52 |
| CHEMBL489551 | — | — | 1 | 4.51 |
| CHEMBL4566953 | — | — | 1 | 4.50 |
| CHEMBL237111 | AKBA | -1.0 | 1 | 4.45 |
| CHEMBL399873 | UVAOL | — | 1 | 4.38 |
| CHEMBL395428 | — | — | 1 | 4.38 |
| CHEMBL464466 | ARJUNOLIC ACID | — | 1 | 4.38 |
| CHEMBL487933 | — | — | 1 | 4.38 |
| CHEMBL455098 | beta-AMYRIN | — | 1 | - |
| CHEMBL4462866 | — | — | 1 | - |
| CHEMBL400074 | ERYTHRODIOL | — | 1 | - |
| CHEMBL168 | OLEANOLIC_ACID | — | 1 | - |
| CHEMBL201515 | MASLINIC ACID | — | 1 | - |
| CHEMBL486400 | HEDERAGENIN | — | 1 | - |
| CHEMBL1797308 | — | — | 1 | - |
| CHEMBL2386825 | — | — | 1 | - |
| CHEMBL461060 | — | — | 1 | - |
| CHEMBL1802044 | — | — | 1 | - |
| CHEMBL455357 | — | — | 1 | - |
| CHEMBL518475 | — | — | 1 | - |
| CHEMBL169 | URSOLIC ACID | 2.0 | 1 | - |
| CHEMBL391533 | COROSOLIC ACID | — | 1 | - |
| CHEMBL404313 | ASIATIC ACID | — | 1 | - |
| CHEMBL267225 | BOSWELLIC ACID | 2.0 | 1 | - |
| CHEMBL2386654 | — | — | 1 | - |
| CHEMBL236906 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL93 | ZILEUTON | IC50 | = | 23900.0 | nM | 4.62 |
| CHEMBL230006 | ENOXOLONE | IC50 | = | 24800.0 | nM | 4.61 |
| CHEMBL4561483 | — | IC50 | = | 25700.0 | nM | 4.59 |
| CHEMBL437964 | 11-KETO BOSWELLIC ACID | IC50 | = | 28300.0 | nM | 4.55 |
| CHEMBL520726 | — | IC50 | = | 30400.0 | nM | 4.52 |
| CHEMBL489551 | — | IC50 | = | 30600.0 | nM | 4.51 |
| CHEMBL4566953 | — | IC50 | = | 31800.0 | nM | 4.50 |
| CHEMBL237111 | AKBA | IC50 | = | 35600.0 | nM | 4.45 |
| CHEMBL399873 | UVAOL | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL395428 | — | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL464466 | ARJUNOLIC ACID | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL487933 | — | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL455098 | beta-AMYRIN | IC50 | > | 40000.0 | nM | - |
| CHEMBL4462866 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL400074 | ERYTHRODIOL | IC50 | > | 40000.0 | nM | - |
| CHEMBL168 | OLEANOLIC_ACID | IC50 | - | — | - | |
| CHEMBL201515 | MASLINIC ACID | IC50 | > | 40000.0 | nM | - |
| CHEMBL486400 | HEDERAGENIN | IC50 | > | 40000.0 | nM | - |
| CHEMBL1797308 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL2386825 | — | IC50 | - | — | - | |
| CHEMBL461060 | — | IC50 | - | — | - | |
| CHEMBL1802044 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL455357 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL518475 | — | IC50 | - | — | - | |
| CHEMBL169 | URSOLIC ACID | IC50 | > | 40000.0 | nM | - |
| CHEMBL391533 | COROSOLIC ACID | IC50 | > | 40000.0 | nM | - |
| CHEMBL404313 | ASIATIC ACID | IC50 | > | 40000.0 | nM | - |
| CHEMBL267225 | BOSWELLIC ACID | IC50 | > | 40000.0 | nM | - |
| CHEMBL2386654 | — | IC50 | > | 40000.0 | nM | - |
| CHEMBL236906 | — | IC50 | > | 40000.0 | nM | - |