Assay Detail
Binding
CHEMBL4417859
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal hexa-histidine-tagged wild type DUSP5 phosphatase domain (unknown origin) expressed in Escherichia coli BL21 (DE3) incubated for 90 mins in presence of Triton-X100 by pNPP phosphatase assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Dual specificity protein phosphatase 5 (CHEMBL1250380) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Small molecule antagonists of dusp5 and methods of use
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.21 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL179166 | SODIUM ORTHOVANAD | — | 1 | 8.10 |
| CHEMBL4448902 | — | — | 1 | 4.58 |
| CHEMBL4476561 | — | — | 1 | 4.52 |
| CHEMBL4442649 | — | — | 1 | 4.48 |
| CHEMBL4453621 | — | — | 1 | 4.36 |
| CHEMBL316894 | — | — | 1 | - |
| CHEMBL4533219 | — | — | 1 | - |
| CHEMBL4476403 | — | — | 1 | - |
| CHEMBL4463659 | — | — | 1 | - |
| CHEMBL472004 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL179166 | SODIUM ORTHOVANAD | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL4448902 | — | IC50 | = | 26400.0 | nM | 4.58 |
| CHEMBL4476561 | — | IC50 | = | 30000.0 | nM | 4.52 |
| CHEMBL4442649 | — | IC50 | = | 33000.0 | nM | 4.48 |
| CHEMBL4453621 | — | IC50 | = | 44000.0 | nM | 4.36 |
| CHEMBL316894 | — | IC50 | = | 6400000.0 | nM | - |
| CHEMBL4533219 | — | IC50 | = | 5100000.0 | nM | - |
| CHEMBL4476403 | — | IC50 | > | 1000000.0 | nM | - |
| CHEMBL4463659 | — | IC50 | = | 16000000.0 | nM | - |
| CHEMBL472004 | — | IC50 | = | 1700000.0 | nM | - |